Part:BBa_M36019:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M36019

Stanford Location By: Melissa, Sydney, Rachel (BIOE 44 Fall 2014) Experience:

From fluorescence assay conducted, we found that the sensor failed to perform its intended function (detecting lactic acid). Fluorescence/OD measurement remained constant across lactic acid concentrations on a log scale from 0 to 0.1M. We suspect that it is because E. coli themselves regulate lactate in the environment, hence over time the lactate concentration equilibrates to a constant concentration across all experimental set ups.

- Barcode # 0133010776, 0133010749, 0133024645, 0133009701, 0133009727

- Plasmid name: somcrl1

- Antibiotic resistance: amp

- DNA 2.0 gene # 193463

- Organism expressed in: E. Coli

- Sensor for lactic acid

User Reviews

UNIQ976932d2236bec7c-partinfo-00000000-QINU UNIQ976932d2236bec7c-partinfo-00000001-QINU